By Julie Beal

Is it possible to shed and spread a genetic vaccine? Some of the people who’ve had a coronavirus vaccine seem to think so, despite being ‘pro-vax’. Most of them are simply saying what they’ve experienced, not making accusations about the vax being spread.

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Journalists have been quick to claim it’s not possible to shed the vaccine; they’re taking their lead from Pfizer who’ve said it can’t be shed because it doesn’t contain a live virus. However, there is strong evidence that vaccine components can be shed from the body and spread to another person, even though they don’t contain a live virus. It’s not known how much this can happen, but clearly breast-milk and semen are like strong intravenous doses. It also appears that vax makers know about this. For example, Moderna has published several mRNA-based patents in which they describe analysing exosomes released from a subject, to test how well their product is working. Exosomes are tiny particles that are constantly coming out of our cells like a cloud of dust, and they’re found in every type of bodily fluid you could ever imagine. It seems they can also contain components of genetic vaccines which are then capable of having a biological impact.

To explore the issue further, this article will examine some of the available evidence about what happens to mRNA LNPs once they’re inside a cell, drawing on knowledge gained from the growing body of research on exosomes. It will then look at how vaccine-viruses can be spread when replicating viruses are used, and conclude by trying to make sense of the Pfizer trial protocol.
MODERNA’S EXOSOMES

According to several Moderna patents (including the very recent ‘RSV RNA Vaccines’, dated 2021), the successful delivery of their mRNA product can be assessed by examining exosomes that have come from either body organs or bodily fluids such as blood, sweat and tears. A sample of around 2 mL can be obtained from the subject in order to do this, and there’s a very long list of bodily fluids to choose from:

“…. the nucleic acids of the present invention may be quantified in exosomes or when derived from one or more bodily fluid. Bodily fluids include peripheral blood, serum, plasma, ascites, urine, cerebrospinal fluid (CSF), sputum, saliva, bone marrow, synovial fluid, aqueous humor, amniotic fluid, cerumen, breast milk, broncheoalveolar lavage fluid, semen, prostatic fluid, cowper’s fluid or pre-ejaculatory fluid, sweat, fecal matter, hair, tears, cyst fluid, pleural and peritoneal fluid, pericardial fluid, lymph, chyme, chyle, bile, interstitial fluid, menses, pus, sebum, vomit, vaginal secretions, mucosal secretion, stool water, pancreatic juice, lavage fluids from sinus cavities, bronchopulmonary aspirates, blastocyl cavity fluid, and umbilical cord blood. Alternatively, exosomes may be retrieved from an organ selected from the group consisting of lung, heart, pancreas, stomach, intestine, bladder, kidney, ovary, testis, skin, colon, breast, prostate, brain, esophagus, liver, and placenta.”

So… what are exosomes?

They are tiny particles that bud out of our cells and can travel round the body taking messages to other cells. An exosome is formed inside a cell when various bits and pieces that are hanging around get wrapped up (encased) in a bit of the cell membrane, then pushed out of the cell into the bloodstream. It’s basically a little package of DNA/RNA protected by the lipid membrane it got from the cell. It can also contain proteins. Most of the details have only recently been discovered, but many experts are now saying that exosomes are, in many ways, indistinguishable from enveloped viruses, such as SARS.

“Cells talk in a language that looks like viruses”

Exosomes are similar to many viruses because they are lipid nanoparticles that contain “bioactive materials” and carry information around the body. Also known as extracellular vesicles (EVs), exosomes can only exist if they are created by a cell, and it’s the same for enveloped viruses.

“… extracellular vesicles and enveloped viruses are similar in both composition and function. Their high degree of similarity makes differentiating between vesicles and enveloped viruses in biological specimens particularly difficult.”

After an explosion of research into EVs, they are still not well characterized, but are said to be extremely diverse. Overall, though, a consensus is forming that exosomes, or EVs, form a continuum of various different types, and are therefore very hard to classify into separate groups.

What was Moderna looking for?

Moderna often refer to their mRNA constructs as “chimeric polynucleotides”. This term is very apt because it reflects the fact that they are really rather different to normal mRNA produced by the natural world. The patents describe isolating exosomes in order to quantify the amount of chimeric polynucleotides they contain, to help determine how much of the mRNA got turned into proteins (translation); it also demonstrates changes to the original sequences, e.g. bits going missing (truncation). These sequences can easily be identified as being Moderna ones, because they are extensively modified and distinctly different to normal RNA produced by natural living beings. This is how it’s described:

“In the analysis, the level or concentration of a chimeric polynucleotide may be an expression level, presence, absence, truncation or alteration of the administered construct. These methods afford the investigator the ability to monitor, in real time, the level of chimeric polynucleotides remaining or delivered. This is possible because the chimeric polynucleotides of the present invention differ from the endogenous forms due to the structural or chemical modifications.”

(Note: ‘endogenous’ means you made it naturally yourself, ‘exogenous’ means it’s from somewhere else!)

These tests were probably useful for Moderna in the early days, when investors were keen to see proof that Moderna could get their mRNA to work. Genes make proteins, so to show this new, modified mRNA could work, they had to prove the sequences could be translated into proteins. There was a long history of mRNA not working because it couldn’t get past all the immune defences, so there was a need to show that the modified version was different.

How does it happen?

Once inside a cell, an mRNA LNP is processed in the endosome, which is like a sorting mechanism for stuff that’s been taken up by the cell. Some of this modified mRNA will end up being translated after escaping the endosome, i.e. some spike proteins will get made. If, however, some of the modified mRNA got wrapped up in a bit of the cell’s membrane, it would get pushed out of the cell as an exosome, or EV.

After being ‘excreted’ from the cell in the form of an EV, the mRNA would be able to survive the journey through the blood once again. This time, instead of being encased in Moderna’s nano-sized lipid membrane, it would be encased in the nano-sized lipid membrane it got from the cell. The membrane protects it from being degraded by enzymes such as RNase. Previous research has shown that (natural) RNA and proteins are contained in exosomes derived from serum, plasma, urine, and saliva; they’re able to survive and not get degraded by enzymes because they’re “contained and protected in membrane-bound structures”. This is demonstrated by the fact that, “the RNA contained within exosomes remains amplifiable, implicating protection from RNase degradation by the exosome membrane.” As explained by Sasha Vlassov at ISEV13, nucleic acids will be instantly degraded unless they’re bound to proteins or encapsulated in a membrane:

“There’s no such thing as free-floating DNA or RNA.”